Table 3.
ON | Sequence | B = | DNA: 5′-GTG ABA TGC
|
|||
---|---|---|---|---|---|---|
Tm/°C
|
ΔTm/°C
|
|||||
T | A | C | G | |||
D2 | 3′-CAC TAT ACG | 27.5 | −17.0 | −15.5 | −9.0 | |
W4 | 3′-CAC TWT ACG | 27.5 | −20.0 | −17.0 | −16.0 | |
X4 | 3′-CAC TXT ACG | 34.0 | −16.5 | −7.5 | −17.0 | |
Y4 | 3′-CAC TYT ACG | 39.0 | −21.0 | −12.0 | −17.0 | |
Z4 | 3′-CAC TZT ACG | 35.0 | −21.5 | −14.0 | −14.5 |
For conditions of thermal denaturation experiments, see Table 1. Tm’s of fully matched duplexes are shown in bold. ΔTm = change in Tm relative to fully matched DNA:DNA duplex.