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. Author manuscript; available in PMC: 2014 Dec 20.
Published in final edited form as: J Org Chem. 2013 Dec 11;78(24):12690–12702. doi: 10.1021/jo4022937

Table 4.

Discrimination of mismatched RNA targets by B4-series and reference ONs.a

ON Sequence B = RNA: 5′-GUG ABA UGC
Tm/°C
ΔTm/°C
U A C G
D2 3′-CAC TAT ACG 24.5 −15.0 −15.0 −11.0
W4 3′-CAC TWT ACG 29.0 −13.5 −13.0 −11.5
X4 3′-CAC TXT ACG 24.0 −10.5 −8.0 −10.0
Y4 3′-CAC TYT ACG 27.0 −10.5 −11.0 −8.5
Z4 3′-CAC TZT ACG 31.0 <−19.0 −15.5 −14.5
a

For conditions of thermal denaturation experiments, see Table 1. Tm’s of fully matched duplexes are shown in bold. ΔTm = change in Tm relative to fully matched RNA:DNA duplex.