Table 1.
The top 10 pathogen-associated F. graminearum proteins in terms of cereal and pathogen F-measure
| Protein id | F-measure | Pfam domain (E-value, ID) | Phyre2 structure (Confidence/Coverage) | Molecular weight (kDA) | Signal peptide | Distribution of significant NR phmmer hits |
|---|---|---|---|---|---|---|
| |
Cereal |
|
|
|
|
|
| FGSG_03333 |
0.71 |
Haloacid dehalogenase-like hydrolase (8e-10, PF12710) |
Phosphorylcholine phosphatase (100%/90%) |
41.1 |
Yes |
93% Bacteria, 7% Ascomycota |
| FGSG_03338 |
0.7 |
– |
Serine protease inhibitor 1 (98.4%/52%) |
14.4 |
No |
100% Ascomycota |
| FGSG_09148 |
0.69 |
Peptidase C69 (3.1e-25, PF03577) |
Acyl-coenzyme (99.2%/44%) |
57.2 |
Yes |
90% Bacteria, 9% Eukaryota, 1% Archaea |
| FGSG_03339 |
0.66 |
– |
Serine protease inhibitor 1 (99.2%/55%) |
14.7 |
No |
100% Ascomycota |
| FGSG_09328 |
0.66 |
Fungal specific transcription factor domain (3.5e-05, PF04082) |
Centromere DNA-binding protein complex cbf3 (98.2%/72%) |
62.6 |
No |
94% Ascomycota, 6% Basidiomycota |
| FGSG_04015 |
0.66 |
– |
– |
57.4 |
No |
100% Ascomycota |
| FGSG_03861 |
0.64 |
DUF3425 (1.8e-14, PF11095) |
Pyrimidine pathway regulator 1 (99.4%/15%) |
62.9 |
No |
94% Ascomycota, 5% Basidiomycota, 1% others |
| FGSG_04507 |
0.63 |
C2 domain (1.1e-10, PF00168) |
Endocytosis, exocytosis, synaptotagmin-1 (100%/54%) |
52.1 |
No |
41% Metazoan, 29% Viridiplantae, 22% Ascomycota, 8% others |
| FGSG_07909 |
0.62 |
Homeobox KN domain (2.3e-15, PF05920) |
Homeobox domain (99.7%/11%) |
84.6 |
Yes |
56% Metazoan, 29% Viridiplantae, 12% Ascomycota, 3% others |
| FGSG_07846 |
0.61 |
FMO-like (5.8e-16, PF00734) |
Monooxygenase (100%/75%) |
62.6 |
No |
37% Bacteria, 32% Metazoan, 15% Viridiplantae, 12% Ascomycota, 4% others |
| |
Pathogen |
|
|
|
|
|
| FGSG_04060 |
0.65 |
Rare lipoprotein A like double-psi beta barrel (3.1e-05, PF03330) |
Beta-expansin 1a (100%/94%) |
22.2 |
Yes |
48% Bacteria, 16% Ascomycota, 13% Basidiomycota, 8% Phytophthora, 5% dictyostelium, 10% others |
| FGSG_09841 |
0.65 |
– |
– |
20.8 |
Yes |
76% Ascomycota, 15% Bacteria, 6% Archaea, 3% others |
| FGSG_11496 |
0.64 |
Rare lipoprotein A like double-psi beta barrel (3.1e-05, PF03330) |
Beta-expansin 1a (100%/93%) |
25.2 |
Yes |
23% Viridiplantae, 21% Bacteria, 18% Ascomycota, 13% Basidiomycota, 11% Phytophthora, 6% Dictyostelium, 8% others |
| FGSG_03333 |
0.58 |
Haloacid dehalogenase-like hydrolase (8e-10, PF12710) |
Phosphorylcholine phosphatase (100%/90%) |
41.1 |
Yes |
93% Bacteria, 7% Ascomycota |
| FGSG_09148 |
0.55 |
Peptidase C69 (3.1e-25, PF03577) |
Acyl-coenzyme (99.2%/44%) |
57.2 |
Yes |
90% Bacteria, 9% Eukaryota, 1% Archaea |
| FGSG_04507 |
0.52 |
C2 domain (1.1e-10, PF00168) |
Endocytosis, exocytosis, synaptotagmin-1 (100%/54%) |
52.1 |
No |
41% Metazoan, 29% Viridiplantae, 22% Ascomycota, 8% others |
| FGSG_03549 |
0.52 |
– |
– |
28.2 |
No |
100% Ascomycota |
| FGSG_11152 |
0.49 |
– |
Coronatine-insensitive protein 1 (99.9%/94%) |
44.4 |
No |
100% Ascomycota |
| FGSG_09328 |
0.48 |
Fungal specific transcription factor domain (3.5e-05, PF04082) |
Centromere DNA-binding protein complex cbf3 (98.2%/72%) |
62.6 |
No |
94% Ascomycota, 6% Basidiomycota |
| FGSG_07909 | 0.48 | Homeobox KN domain (2.3e-15, PF05920) | Homeobox domain (99.7%/11%) | 84.6 | Yes | 56% Metazoan, 29% Viridiplantae, 12% Ascomycota, 3% others |
For each protein, its Pfam annotation, Phyre2 structure prediction, molecular weight and signal peptide predicted by SignalP are given as well as the distribution of significant phmmer hits against the non-redundant protein databank (NR) in terms of taxonomy.