Skip to main content
. 2013 Dec 5;137(2):420–432. doi: 10.1093/brain/awt327

Figure 1.

Figure 1

Loss of merlin in sciatic nerve lysates is accompanied by decreasing NRG1 type III levels. (A–F) Immunoblots of sciatic nerve lysates taken from adult mice. (A) Loss of each major merlin isoform in transgenic animals (nf2 iso1 KO; nf2 iso2 KO) results in decreased expression of NRG1 type III and reduced Akt phosphorylation (p-Akt). Actin was used as loading control (n = 3). (B) Loss of merlin-iso1 or merlin-iso2 in vivo leads to reduced expression of NOTCH1 (n = 2). (C and D) Schwann cell-specific loss of merlin (P0-Cre;Nf2fl/fl) has no effect on NRG1 type III level but lowers NOTCH1 expression compared with wild-type littermates (P0-Cre;Nf2+/+; n = 3). (E and F) Neuron-specific merlin knockout (Nefh-Cre;Nf2floxfl/fl) reduces levels of NRG1 type III and p-Akt. NOTCH1 levels are unchanged in sciatic nerves after loss of merlin in the neuronal compartment (n = 2). (G) Isoform-specific loss of merlin reduces NRG1 type III amounts in hind-brain lysates of adult mice (n = 3). Blot quantifications (density values) are depicted below respective lanes and are normalized to actin and wild-type controls. KO = knockout.