Figure 8.

Gene set enrichment analysis (GSEA) and identification of L-GMP-specific transcripts.

1. GSEA showing significant enrichment (p-value 0.0215) of the top 150 murine ST-GMP-associated gene set differentially expressed between human t(8;21) CBF AML patients and non-leukaemic controls.

2. GSEA showing enrichment of the top 150 murine L-GMP-associated gene set differentially expressed between human t(8;21) CBF AML patients and non-leukaemic controls. In A and B the enrichment plot is shown at the top. Black vertical lines indicate gene hits and the ranking metric scores based on a t-test analysis are depicted as a grey line plot. NES, normalized enrichment score.

3. Gene expression heat map of 17 L-GMP-specific genes with each column representing the difference in expression between median transcription values in 61 t(8;21) CBF AML patients (red lane), sorted primary human CD34⁺/CD38⁻ cord blood HSC (HSC1), CD34⁺/CD38^low/CD36⁻ cord blood HSC (HSC2), primary human CD34⁺/CD38⁺ haematopoietic progenitors (Progenitors) and unsorted Lin^+/− human primary cord blood cells (Whole blood), obtained from previously published micro-array data (Eppert et al, 2011). Relative transcription values are colour coded from red (high) through yellow (intermediate) to green (low).

4. Gene expression heat map of 17 L-GMP-specific genes with each column representing the difference in expression between transcription values. The first two columns show median transcription values for 61 t(8;21) patients and 69 healthy control donors. On the right 61 individual gene transcription profiles from t(8;21) AML biopsies (t(8;21) patients) and 69 individual gene transcription profiles from healthy donors (Controls) are shown. Relative transcription values are colour coded from red (high) through yellow (intermediate) to green (low).

5. Expression of 17 L-GMP-specific genes in normal human embryonic and adult tissues. Relative transcription values are colour coded from black (expressed) through grey (intermediate) to white (absent).