Figure 7.

Rcan1 re-expression in Apoe^−/−Rcan1^−/− macrophages increases CD36 expression and the uptake of oxLDL Apoe^−/−Rcan1^−/− peritoneal macrophages were transduced with lentiviruses encoding GFP or Rcan1-1-IRES-GFP plus Rcan1-4-IRES-GFP.

• A. Representative immunoblot analysis of Rcan1 expression in these cells.

• B. Quantification of the normalized median fluorescence levels of CD36 determined by flow cytometry in the GFP⁺ population (n = 4; mean ± SEM; Student's t-test *p = 0.02).

• C,D. (C) Representative Laurdan GP images and (D) quantification of the area covered by oxLDL (red dots) in GFP-F4/80 double-positive cells after culture in the presence of oxLDL (n = 3; mean ± SEM; Student's t-test, *p = 0.01). Scale bar, 10 µm.