. 2013 Oct 18;5(12):1852–1870. doi: 10.1002/emmm.201202435

Table 2.

Kinetic parameters of methionine sulfoxide reductase activity of MsrA2 or MsrB2

Enzyme–substrate	V_max	K_m	Efficiency (V_max/K_m)
Enzyme–substrate	nmol/mg min	µmol/L	AU
MsrA2 + Etrx1	59.0	26.5	2.22
MsrA2 + Etrx2	8.2	13.3	0.61
MsrB2 + Etrx1	(No activity)	(No activity)	(No activity)
MsrB2 + Etrx2	17.1	21.0	0.81

The methionine sulfoxide reductase activity was measured in the presence of Etrx1 and Etrx2 protein, respectively. The reaction was performed at pH 7.4 in a mixture containing the Etrx protein, NADPH, human thioredoxin reductase and the reaction was started by the addition of MsrAB2 subunits.

AU, arbitrary units.