NAC and NBMI protect against the bleomycin-induced tight junction protein, occludin, alterations in ECs. BPAECs on coverslips (5 × 105 cells/35-mm dish) were subjected to treatment with MEM alone or MEM containing bleomycin (5, 10, and 100 µg) for 4 h (A,B). BPAECs were also pre-treated with MEM or MEM containing 5 mM NAC (C,D) or 50 µM NBMI (E,F) for 1 h and then subjected to co-treatment with MEM containing bleomycin (10 µg) for 4 h. At the end of the incubation period, the cells were fixed and stained as described under Materials and Methods. The fluorescence intensities were measured as described under Materials and Methods. Each micrograph is a representative picture obtained from three independent experiments conducted under identical conditions. Data represent mean ± SD calculated from three independent experiments. *Significantly different at p < 0.05 as compared to cells treated with MEM alone. **Significantly different at p < 0.05 as compared to cells treated with MEM containing bleomycin alone.