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. Author manuscript; available in PMC: 2014 Feb 5.
Published in final edited form as: Toxicol Mech Methods. 2012 Jun;22(5):383–396. doi: 10.3109/15376516.2012.673089

Figure 2.

Figure 2

NAC and NBMI protect against bleomycin-induced morphological alterations in ECs. BPAECs (5 × 105 cells/35-mm dish) were pretreated with MEM or MEM containing the chosen antioxidant (5 mM NAC and 50 µM NBMI) for 1 h and then subjected to co-treatment with MEM containing bleomycin (10 µg) for 12 h to determine the protective effect of the antioxidants on bleomycin-induced cell morphology alterations. At the end of the incubation period, the cell morphology was examined under light microscope (as an index of EC monolayer disruption and cytotoxicity) as described under the section “Materials and Methods”. Each micrograph is a representative picture obtained from three independent experiments conducted under identical conditions.