Table 1.
Conditions for the chitin isolation.
| Assay | Treatment 1 | Treatment 2 | Treatment 3 |
|---|---|---|---|
| A1 | Dried and powdered biomass | Deproteinization NaOH 1 M 1 : 30 (w/v) 40°C, 2 h |
|
| A2 | Dried and powdered biomass | Deproteinization NaOH 1 M, 2 M 1 : 30 (w/v) 90°C, 2 h |
|
| A3 | Dried and powdered biomass | Deproteinization NaOH 2 M, 4 M 1 : 30 (w/v) 90°C, 2 h |
Decolorization KMnO4
10 g L−1, 1 h C2H2O4 10 g L−1, 1 h |
| A4 | Dried and powdered biomass, immersion in hot water in order to remove polysaccharides | Deproteinization NaOH 2 M, 4 M, 6 M, and 8 M 1 : 20 (w/v) 100°C, 3 h |
Decolorization KMnO4
10 g L−1, 1 h C2H2O4 10 g L−1, 1 h |
| A5 | Dried and powdered biomass and sonication 40 min washed with ethanol 24 h |
Deproteinization NaOH 4 M 1 : 20 (w/v) 100°C, 3 h Three times. |
Decolorization KMnO4 10 g L−1, 1 h C2H2O4 10 g L−1, 1 h |