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. 2013 Dec 6;196(2):397–401. doi: 10.1534/genetics.113.160135

Figure 2.

Figure 2

Southern blot hybridization analysis of the centromeric satellite repeats in different potato cultivars. (A) Southern blot hybridization analysis of the Cen2-specific satellite repeat St3.58 in four potato cultivars. The numbers in the parentheses indicate the copy number of chromosome 2 carrying St3.58 in each cultivar. Left: genomic DNAs from the four cultivars were digested by BamHI and separated by a 0.8% agarose gel. Right: blotted DNAs were hybridized to St3.58 (plasmid probe 1331). No hybridization was found to DNA of White Pearl. (B) Southern blot hybridization analysis of the Cen1-specific satellite repeat St24 in four potato cultivars. The numbers in parentheses indicate the copy number of chromosome 2 carrying St24 in each cultivar. Left: genomic DNAs from the four cultivars were digested by HindIII and separated by a 0.8% agarose gel. Right: blotted DNAs were hybridized to St24 (plasmid probe 1336). No hybridization was found to DNA of Superior. Southern blot hybridization followed published protocol (Stupar et al. 2002).