Figure 3.
FISH mapping of the satellite repeats St24 and St57 in tetraploid potato genotypes. (A–D) FISH mapping of the Cen1-specific satellite repeat St24 in four potato cultivars, Hindenburg, Kennebec, Juanita, and Superior, respectively. Potato chromosome 1 was identified by bacterial artificial chromosome clone 96H03 (red signals). Arrows point to strong FISH signals and arrowheads point to weak FISH signals. (A′–D′) The FISH signals from St24 were digitally separated from A to D, respectively. (E–H) FISH mapping of the Cen7-specific satellite repeat St57 in four potato cultivars, White Pearl, MegaChip, Kerr’s Pink, and Katahdin, respectively. Potato chromosome 7 was identified by bacterial artificial chromosome clone 186I02 (red signals). Arrows point to strong FISH signals and arrowheads point to weak FISH signals. (E′–H′) The FISH signals from St57 were digitally separated from E to H, respectively. Plasmids 1336 and 1338 were used to detect repeats St24 and St57, respectively. Bars, 5 µm.