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. 2013 Dec 13;196(2):443–453. doi: 10.1534/genetics.113.159541

Figure 6.

Figure 6

dASCIZ is required for cell survival through Ctp. (A and B). Basal sections of wing imaginal discs, showing extruded centrosomin-positive mitotic cells in the disc expressing En > dASCIZ RNAi (B) but not in the control (A), costained for DNA with DAPI. (C) Quantification of the total number of centrosomin-positive cells extruded below the basal lamina after expression of dASCIZ RNAi in the PC (P = 0.0003). Error bars represent SEM for n = 6. (D) PC/AC ratio of caspase staining in the wing imaginal disc. Error bars represent SEM for n = 8. Ratio is increased for dASCIZ RNAi (P = 0.0195) and Ctp RNAi (P = 0.0001). Coexpression of dASCIZ RNAi with UAS–Ctp improves the ratio compared to dASCIZ RNAi alone (P = 0.0064) back to control levels (P = 0.3439). (E–L) Basal sections of wing imaginal discs stained with the activated caspase 3 antibody to detect apoptosis (left), and DAPI-stained pyknotic cells are marked with arrowheads (right). The En–GAL4 expression area in the PC is outlined in white. (E and F) En–GAL4/+ control. (G and H) En > dASCIZ RNAi. (I and J) En > Ctp RNAi. (K and L) En > dASCIZ RNAi with UAS–Ctp overexpression.