Effects of high Rho1 activity on Spg1 activation in Etd1-depleted cells. Cdc7-Tom was imaged by time-lapse microscopy at 5-min intervals in living wild-type (wt), etd1Δ, wt/p41rho1 (nmt41x-driven expression of rho1 in plasmid pREP41x), etd1Δ/p41rho1, rga5Δ, and etd1Δ rga5Δ cells under restrictive conditions for the etd1Δ mutation (25°). Fluorescence intensity was quantified (arbitrary units) and represented for each SPB (SPBd and dSPBm).