Table 1.
Antibody | Immunogen | Working dilution (EM) | Working dilution (LM) | Source |
---|---|---|---|---|
Anti-VIAAT | Amino acids 2-115 of rat VIAAT | 1:100 | 1:500 | Rabbit polyclonal (Synaptic Systems 131013) |
Anti-GAD65 | Affinity-purified GAD65 from rat brain | 1:40 | 1:100 | Mouse monoclonal (DSHB GAD-6) |
Anti-gephyrin | Purified gephyrin from rat | 1:50 | 1:250 | Mouse monoclonal (Synaptic Systems 147021) |
Anti-GABAAR α2 | Amino acids 29-37 of rat GABAARα2 precursor protein | — | 1:500 | Rabbit polyclonal (Synaptic Systems 224103) |
EM: electron microscopy, LM: light microscopy.