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. 2014 Feb 1;4:4. doi: 10.1186/2044-5040-4-4

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Copyright © 2014 Bosnakovski et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Development of the primary assay. (A) Cell death of iC2C12-DUX4 cells 36 h after addition of 500 ng/mL doxycycline. (B) Optimization of cell number and dox concentration. The clearest difference between viability of control and DUX4-induced cells was seen at 1,000 cells per well with 500 ng/mL dox. n = 6. Error bars represent SEM.