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. 2014 Jan 6;6(1):179–191. doi: 10.1093/gbe/evu002

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© The Author(s) 2014. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3.— — Myo-sex hybridizes to the location of the A. aegypti M-locus and can still recombine with the m-chromosome. (A) Using two transgenic strains of A. aegypti with sex-linked insertions, sensor and J2, a transgenic strain of A. aegypti was generated that has transgenes flanking the M-locus on the M-chromosome, and a separate transgenic strain was generated with the two transgenes corresponding positions on the m-chromosome. Note that one of the 43 transgenic recombinants died before we can determine its sex. (B) A representation of the relative position of the transgenes with respect to the M-locus and the m-locus. Numbers represent linkage in centimorgans. FISH on mitotic chromosomes from the kh^w strain of A. aegypti in males (C) and females (D) with probes for J2 and myo-sex. In (C), the blue J2 signal spans the entire presumed M-locus and the surrounding region. (E) FISH on male polytene chromosome shows that the myo-sex signal is fully within the J2 signal, indicating that myo-sex is located between the J2 and sensor transgenes. Myo-sex was present in the transgenic strain with the transgenes on the m-chromosome. When J2 recombined with the m-chromosome to generate this transgenic line, myo-sex also moved to the m-chromosome with J2 indicating that myo-sex can still recombine.