Fig. 4.—

Myo-sex expression. (A) The expression profile of myo-sex from RT-PCR on cDNA spanning developmental time points. PCR products indicate expression of myo-sex in the larva, pupa, and adult male. A ribosomal protein gene (RPS7) was amplified in both male and females samples to verify the integrity of the cDNA. (B) RT-PCR on males and recombinant females. cDNA were synthesized from ten pooled individuals in each sample. The recombinant females were the m/m^J2sensor females shown in figure 3A. Thirty-two cycles of amplification were performed.