TABLE 1.
CO Dissociation Constants for sGC Proteins
| Protein | α1 | β1 | KdCO (μM) | KdCO′ (μM, +Ligand) | Ref. | |
|---|---|---|---|---|---|---|
| Bt sGC (full length) | 1–691 | 1–619 | 127 | ~26 | YC-1 | 51 |
| 97 | 76 | |||||
| Hs sGC (full length) | 1–690 | 1–619 | 260 | 77 | ||
| Ms sGC-NT2 | 49–471 | 1–401 | 77 ± 7 | 1.7 ± 0.1 | YC-1 | 33 |
| 90 ± 9 | 1.0 ± 0.1 | YC-1 | 43 | |||
| Ms sGC-NT13 | 49–450 | 1–380 | 2.8 ± 0.4 | YC-1 | ||
| 53 ± 4 | 2.9 ± 0.2 | PF-25 | This work | |||
| 0.25 ± 0.02a | BAY | |||||
| Ms sGC-NT19 | 49–450 | 1–380 | 50 ± 3 | 0.8 ± 0.1 | YC-1 | 43 |
| Ms sGC-NT21 | 272–450 | 1–380 | 0.20 ± 0.02a | YC-1 | ||
| 2.2 ± 0.2a | 0.24 ± 0.01a | PF-25 | This work | |||
| 0.07 ± 0.01a | BAY | |||||
| Ms sGC β1(1–380) | absent | 1–380 | 0.20 ± 0.03a | 0.18 ± 0.05a | YC-1 | This work |
| Bt sGC β1(1–197) | absent | 1–197 | 1.6 ± 0.2b | 1.2 ± 0.2b | YC-1 | This work |
| Bt sGC β1(1–359) | absent | 1–359 | 15 ± 4 | 10 ± 3 | YC-1 | This work |
Titration binding data were measured using gas-tight syringes and 1 or 10 cm cuvettes fitted with rubber septum. Protein concentration was 1 μM unless otherwise indicated. Where included for measuring KdCO′, the YC-1 and PF-25 concentrations were 50 μM, and BAY 41-2272 concentrations were 2.5 μM (Ms sGC-NT21) or 10 μM (Ms sGC-NT13). The values listed are the mean and standard deviation of at least three independent measurements.
a
Measured in a 10 cm cuvette, using 0.1 μM protein.
b
Measured in a 1 cm cuvette, using 0.5 μM protein.