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Cooperativity Factors for sGC Proteins.
|
Kint (CO)a
|
KdCO/KdCO′
|
KdPF-25/KdPF-25′ (NO)
|
|||||
|---|---|---|---|---|---|---|---|
| Protein | YC-1 | PF-25 | BAY | YC-1 | PF-25 | BAY | PF-25 |
| Ms sGC-NT13 | 19 ± 2 | 53 ± 3 | N.M.b | 19 ± 3 | 18 ± 2 | 212 ± 23 | |
| Ms sGC-NT21 | 13.8 ± 0.3 | 19 ± 4 | 58 ± 7 | 11 ± 1 | 9 ± 1 | 31 ± 5 | 14 ± 3 |
| Ms sGC β1(1–380) | 13 ± 2 | ||||||
From global fitting.
Could not be readily measured by global fitting due to the large cooperativity factor, requiring both 1 and 10 cm cuvettes be used.
