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. 2014 Feb 5;9(2):e86815. doi: 10.1371/journal.pone.0086815

Figure 2. Morphometric analysis assessed islets from HF fed BL6J.

Figure 2

(A) Total β cell area per pancreas, (B) β cell mass, and (C) average area of an individual islet in pancreatic sections from mice on normal rodent chow (NC) and high fat (HF) diet were compared The distribution of area of an individual islet is shown in (D). 6 sections for NC and 5 sections for HF fed mice were analyzed for (A–B). 4 sections per group from NC and HF fed mice with total of 448 islets for NC and 653 islets for HF were analyzed for (C–D). (E–H) The percentage of β cells positive for anti BrdU antibody was compared between NC and HF fed mice labeled with BrdU at 1 month of age (E), and 4 month of age (F). 4 sections per group were analyzed for (E) and 5 sections per group were analyzed for (F). Representative photos from an islet labeled at 1 month of age on NC (G) and HF (H) are shown. Green staining: BrdU. Red staining: insulin. Scale bars indicate 50 µm. Data are mean ± s.e.m., n = 4–5 for (E) and (F). ** p<0.01 vs. NC, *** p<0.005 vs. NC.