Table 2. [Ca²⁺]_i in FL cells (FI₅₂₆).

	Sham	EGF	MF
Mean	30.55	38.34	39.87
Error (SD)	1.04	1.53	4.09

After fixed in 4% paraformaldehyde at 4°C overnight, FL cells were stained with 24.8 µg/ml Fluo-3/AM(EX/EM: 488 nm/525 nm) for 40 min at 37°C in the dark. A 0.5-ml (1×10⁵/ml) cell suspension was collected for each group for flow cytometry measurements. The detailed information of experimental conditions and repeating numbers of samples is seen in Table 1.