Figure 8. Silencing down Tregs SENEX gene led to upregulation of P53, P16, P21 and Caspase-3 mRNA expression in response to stress.

Gene mRNA levels were detected by real-time quantitative PCR. After SENEX gene was silenced down for 24 hours, followed by H₂O₂ treatment for 2 hours, apoptosis regulatory genes Caspase-3, P53, P16 and P21 mRNA expression (2^−△△CT = 6705.73±1124.07, 253.08±16.01, 154.58±35.12 and 5135.79±985.47, respectively) upregulated in CD4+CD25^hi Tregs (p<0.05 for each Mann-Whitney U Test). Data are expressed as the mean ± SD. ▴ vs. H₂O₂-treated cells, P<0.05.