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. 2014 Feb 5;9(2):e87882. doi: 10.1371/journal.pone.0087882

Figure 5. Reduced Rab7 expression results in increased c-met expression and enhanced signaling.

Figure 5

A) Nontarget (NT) vector control or Rab7 shRNA expressing DU145 cells were exposed to HGF for the indicated times. Whole cell lysates were collected and c-met, Akt, and Erk phosphorylation was detected by Western blotting. B) Cells were treated with HGF for the indictated time periods and levels of total c-Met protein were determined by Western blot analysis. Densitometry from three independent experiments was used for graphical representation of c-Met loss over time. C) Cells were treated with the indicated concentrations of HGF for 30 minutes followed by Western blot analysis of the indicated proteins. D) Protein lysates of xenograft tumors were harvested and levels of total c-met and Rab7 protein were detected by Western blot. The bar graph represents the average c-met and Rab7 expression of seven tumors per treatment group as determined by densitometry of Western blot. * = p<0.0007 compared to NT shRNA Rab7. ** = p<0.03 compared to NT shRNA c-met. Error bar represent SEM.