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. 2014 Feb 5;9(2):e87868. doi: 10.1371/journal.pone.0087868

Figure 4. Effect of α-Solanine on expression of metastasis associated molecule.

Figure 4

(A) The mRNA expression of MMP-2/9, EMMPRIN, CD44, ENOS and Ecadherin was presented as means ± S.E.M. of three independent experiments.(B) The expressions of MMP-2 and MMP-9 protein were analyzed by Western blot.(C) The expressions of Ecadherin protein was performed by Western blot.(D) Quantification of the western blot results were performed by calculating the ratio of the value to the control group.(E) The activity of MMP-2 and MMP-9 were analyzed by Gelatin Zymography. (F) Quantification of the Gelatin Zymography results were performed by calculating the ratio of the value to the control group. *p<0.05, **p<0.01, compared with the control. GAPDH was used as a loading control.