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. 2014 Feb 5;9(2):e88021. doi: 10.1371/journal.pone.0088021

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© 2014 Gomez et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Histomorphometric measurement of collagen content after Masson’s Trichrome staining (A) in human small and large diameter varicosities (paired SDv and LDv, n = 5) and healthy saphenous veins (magnificence 4X and 10X) (SV, n = 3). (B) Values obtained after Photoshop measurement of colour density. Values are normalized by total surface area; * P<0.05 as determined by one-way-ANOVA followed by the Tukey post-hoc test and by a paired t-test for varicose veins.