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. 2014 Feb 5;9(2):e88208. doi: 10.1371/journal.pone.0088208

Figure 6. In vivo interaction of Ve proteins with the eLRR-RLK SOBIR1.

Figure 6

(A) Schematic representations of transgenically expressed Ve1 (Ve1HA) and the truncated protein encoded by Δ [30] Ve1. (B) Typical appearance of tobacco leaves upon coexpression of Ave1 and Δ[30]Ve1. Pictures were taken at five days post infiltration, and show representative results for least three independent co-infiltrations. (C) Immunoprecipitation of protein extracts from N. benthamiana or N. tabacum. SlSOBIR1-Myc is copurified with Ve1-eGFP and Ve2-eGFP upon immunoprecipitation with GFP-Trap beads (α-GFP). The eLRR-RLK SlFLS2-eGFP that does not interact with SlSOBIR1 is shown as a control [37]. (D) Upon immunoprecipitation of protein extracts from N. tabacum using α-HA affinity matrix and GFP-trap beads, SlSOBIR1-Myc co-purifies with Ve1HA, Ve2HA and Δ[30]Ve1, whereas no signal is observed upon SlFLS2-eGFP purification. IP: immunoprecipitation; CE: crude extract.