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. 2014 Feb 5;9(2):e88208. doi: 10.1371/journal.pone.0088208

Figure 7. C-terminal cytoplasmic tail is required for Ve1-mediated resistance.

Figure 7

(A) Schematic representations of transgenically expressed Ve1 (Ve1HA) and Ve2 (Ve2HA) and the proteins encoded by the truncated/chimeric genes Ve1ΔCT, Ve1_Ve2CT, Ve2Δ91 and Ve2_Ve1CT. (B) C-terminal cytoplasmic tail is functional required for Ve1-mediated resistance, while Ve2 C-terminus is not functional. C-terminal truncated proteins and chimeras containing the Ve2 C-terminus do not induce HR upon coinfiltration with Ave1. Pictures were taken at five days post infiltration, and show representative leaves for least two independent co-infiltrations. (C) Stability of truncated or chimeric Ve proteins is shown by immunoblotting using HA antibody (α-HA). Coomassie-stained blots (CBS) showing the 50 kDa Rubisco band present in the input samples confirm equal loading.