Figure 1. Dvl2 forms a complex with Sestd1 independent of Dact1 or Vangl2. (A) Sestd1 interacts with Dvl2 in a human cell line. Flag-Sestd1 was expressed with or without HA-Dvl2, protein complexes immunoprecipitated (IP) with anti-FLAG agarose beads, and associated proteins detected by immunoblot (IB) with anti-HA antibody. (B) The interaction between Sestd1 and Dvl2 is independent of Dact1. Flag-Sestd1 or Flag-Sestd1 (1–274) was expressed with or without HA-Dact1 or HA-Dvl2, and co-IP assays performed as in A. (C) Schematic summary of complex formation between Sestd1 and Dvl2. Interacting regions are indicated by gray braces. (D) Schematic diagram of regulatory interactions between the N-terminal and C-terminal halves of the Sestd1 protein inferred from co-IP data with Dvl2, Dact1, and Vangl2.