Figure 2. Dvl2 cooperates with Dact1 and Sestd1 in stimulating cellular Rho GTPase activity. (A) Dvl2, Sestd1 and Dact1 synergistically elevate Rho GTPase activity. The SRE.L reporter and Renilla plasmids were co-transfected into cells alone (–) or with Dvl2, Dact1–2A-Sestd1 (D+S) or both plasmids, and luciferase activity measured 24 h later. The graph represents relative luciferase activity, calculated as the ratio of the firefly to Renilla luciferase activity (mean ± SEM (n = 4)). (B) Dvl2 does not synergize with Sestd1 in Wnt/β-catenin-dependent luciferase reporter assay. The SuperTOP flash reporter and Renilla plasmids were co-transfected into cells alone (–) or with Sestd1, Dvl2, or both, and luciferase activity measured 24 h post-transfection. n.s., not significant (P > 0.05); **, P < 0.01.