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. 2014 Feb 5;9(2):e88168. doi: 10.1371/journal.pone.0088168

Figure 5. Effects of intranasal SKI-II on Nrf2, HO-1 and other inflammatory markers in cigarette smoke exposed mice.

Figure 5

Nrf2 protein level normalized to β-actin in lung. Mice were exposed to smoke only once or 12 times (once daily). The lung was collected 2 h after cigarette smoke exposure. B. HO-1 protein in lung. The Western Blot image from pooled samples was shown. C. MDA in BALF, D. Alveolar macrophage in BALF, E. Neutrophils in BALF, F. CXCL1(KC) in BALF, G. MMP9 in BALF. Mice were treated with SKI-II (10 µM), intranasally before each cigarette smoke. Results are representative of 4–5 independent experiments and are means and S.E. of triplicates.