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. 2014 Feb 5;9(2):e88246. doi: 10.1371/journal.pone.0088246

Figure 5. Appressorium formation and penetration assays of the Δpax1 and Δlrg1 mutants.

Figure 5

A. Appressorium formation on GelBond (GB) hydrophobic surfaces at 25°C for 24 h and appressorium mediated-penetration on onion epidermis (OE) at 25°C for 48 h. The strains were Guy11, Ku80, Ku70, PC20 (Δpax1:PAX1), LR80 (Δlrg1), RC38 (Δlrg1:LRG1), LG25 (Δrga1) and GC22 (Δrga1:RGA1). AP, appressorium; IH, invasive hypha; GM, germ tube; VH, vegetative hypha; B. Appressorium formation induced from mycelium of Ku80, LP55 (Δpax1), LP62 (Δpax1), PC20 (Δpax1:PAX1) on GB surface at 25°C for 24 h. Bars in A and B  =  25 µm.