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. 2014 Feb 5;9(2):e88246. doi: 10.1371/journal.pone.0088246

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© 2014 Li et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Functional analysis of each domain in Lrg1. Either Lrg1-LIM2 or -RhoGAP was essential for appressorium formation, pathogenicity and proper protein localization. Lrg1-LIM1 was only required for Lrg1 localization to septum pores, whereas Lrg1-LIM3 was dispensable for Lrg1 function and localization. CON, conidium; AP, appressorium; PA, pathogenicity assay; B. Functional analysis of each domain in Pax1. Single deletion of the LIM domains in Pax1 didn't lead to any defects in conidiation and pathogenicity, while deletion of the region contained all the three LIM domains resulted in an inability to produce conidia and cause blast disease. CA, Conidiation. Bars = 10 µm.