Table 1. Phenotypic analysis of LIM protein mutants in M. oryzae.
Strain | Growth (cm)a | Conidiation (×104/plate)b | Appresorium (%)c | Penetration(%)d | |
GB | OE | ||||
Guy11 | 6.8±0.1ae | 99.7±6.0a | 97.0±3.6a | 96.7±3.5a | 86.7±3.2a |
Δrga1 | 6.8±0.1a | 95.7±10.7a | 13.3±3.8b | 95.7±2.5a | 83.3±4.5a |
Δrga:RGA1 | 6.8±0.1a | 98.7±8.3a | 96.7±4.2a | 95.0±3.0a | 86.0±4.0a |
Δldp1 | 6.9±0.1a | 93.3±7.6a | 95.7±2.6a | 94.5±4.5a | 85.0±3.0a |
Ku80 | 6.9±0.1a | 93.3±1.5a | 95.7±3.5a | 93.7±2.1a | 82.7±3.1a |
Δpax1 | 3.0±0.1d | 0 | 0 | 0 | 0 |
Δpax1:PAX1 | 5.9±0.1b | 62.3±3.1b | 96.3±1.5a | 91.7±4.a | 82.7±5.0a |
Ku70 | 6.8±0.1a | 95.7±1.5a | 94.7±4.0a | 92.3±4.5a | 82.3±3.1a |
Δlrg1 | 3.5±0.1c | 3.3±1.5c | 0 | 0 | 0 |
Δlrg1:LRG1 | 5.9±0.1b | 65.3±4.5b | 94.7±5.1a | 93.7±3.5a | 84.7±1.5a |
a. The diameter of colonies grown on CM plates at 25°C for 10 d. b. The conidia washed from the 15d-old CM cultures. c. Percentage of appressorium formation on GB (GelBond) surfaces incubated for 24 h or OE (onion epidermis) surfaces incubated for 48 h at 25°C. d. Percentage of invasive hyphae formation from appressoria incubated on OE for 24 h at 25°C. More than 300 spores or appressoria were counted for each strain. e. Different letters after mean values indicated significant difference at P-value of 0.05. Data were calculated from three independent experiments conducted in triplicates. Strains: Guy11, LG25 (Δrga1), GC22 (Δrga:RGA1), LD17 (Δldp1), Ku80, LP55 (Δpax1), PC20 (Δpax1:PAX1), Ku70, LR80 (Δlrg1) and RC38 (Δlrg1:LRG1).