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. 2014 Feb 5;9(2):e88246. doi: 10.1371/journal.pone.0088246

Table 1. Phenotypic analysis of LIM protein mutants in M. oryzae.

Strain Growth (cm)a Conidiation (×104/plate)b Appresorium (%)c Penetration(%)d
GB OE
Guy11 6.8±0.1ae 99.7±6.0a 97.0±3.6a 96.7±3.5a 86.7±3.2a
Δrga1 6.8±0.1a 95.7±10.7a 13.3±3.8b 95.7±2.5a 83.3±4.5a
Δrga:RGA1 6.8±0.1a 98.7±8.3a 96.7±4.2a 95.0±3.0a 86.0±4.0a
Δldp1 6.9±0.1a 93.3±7.6a 95.7±2.6a 94.5±4.5a 85.0±3.0a
Ku80 6.9±0.1a 93.3±1.5a 95.7±3.5a 93.7±2.1a 82.7±3.1a
Δpax1 3.0±0.1d 0 0 0 0
Δpax1:PAX1 5.9±0.1b 62.3±3.1b 96.3±1.5a 91.7±4.a 82.7±5.0a
Ku70 6.8±0.1a 95.7±1.5a 94.7±4.0a 92.3±4.5a 82.3±3.1a
Δlrg1 3.5±0.1c 3.3±1.5c 0 0 0
Δlrg1:LRG1 5.9±0.1b 65.3±4.5b 94.7±5.1a 93.7±3.5a 84.7±1.5a

a. The diameter of colonies grown on CM plates at 25°C for 10 d. b. The conidia washed from the 15d-old CM cultures. c. Percentage of appressorium formation on GB (GelBond) surfaces incubated for 24 h or OE (onion epidermis) surfaces incubated for 48 h at 25°C. d. Percentage of invasive hyphae formation from appressoria incubated on OE for 24 h at 25°C. More than 300 spores or appressoria were counted for each strain. e. Different letters after mean values indicated significant difference at P-value of 0.05. Data were calculated from three independent experiments conducted in triplicates. Strains: Guy11, LG25 (Δrga1), GC22 (Δrga:RGA1), LD17 (Δldp1), Ku80, LP55 (Δpax1), PC20 (Δpax1:PAX1), Ku70, LR80 (Δlrg1) and RC38 (Δlrg1:LRG1).