Table 1. qPCR versus Microarray results correlations and primers used.
Genes | Pearson (r) | Statistics | Foward | Reverse |
Il6 | 0,91 | p = 0,001 | 5′-GTTCTCTGGGAAATCGTGGA-3′ | 5′-TGTACTCCAGGTAGCTATGG-3′ |
Il12a | 0,89 | p = 0,001 | 5′-GATCATGAAGACATCACACGG-3′ | 5′-AGAATGATCTGCTGATGGTGG-3′ |
Il10 | 0,84 | p = 0,01 | 5′-TCAAACAAAGGACCAGCTGGACAACATACTG-3′ | 5′-CTGTCTAGGTCCTGGAGTCCAGCAGACTCAA-3′ |
Il1b | 0,81 | p = 0,01 | 5′-CAGTTCTGCCATTGACCATC-3′ | 5′-TCTCACTGAAACTCAGCCGT-3′ |
Tnfa | 0,82 | p = 0,01 | 5′-TCTCATCAGTTCTATGGCCC-3′ | 5′-GGGAGTAGACAAGGTACAAC-3′ |
Csf2 | 0,80 | p = 0,01 | 5′-TGAACCTCCTGGATGACATG-3′ | 5′-GTGTTTCACAGTCCGTTTCC-3′ |
Cxcl1 | 0.89 | p = 0,001 | 5′- TGAAGCTCCCTTGGTTCAGA-3′ | 5′- AGGTGCCATCAGAGCAGTCT-3′ |
Msr1 | 0.91 | p = 0,001 | 5′- GGGGAGTGTAGGCGGATCAACCCC-3′ | 5′- CGGCCCTCATGGGCTCCACTA-3′ |
Hmox1 | 0.87 | p = 0,001 | 5′- ACCAGAGTCCCTCACAGATGGCG-3′ | 5′- GCAGGGGCAGTATCTTGCACCA-3′ |
β2microglubulin | 0.97 | p = 0,001 | 5′TGACCGGCTTGTATGCTATC-3′ | 5′-CAGTGTGAGCCAGGATATAG-3′ |
Relative differences were calculated according to the delta-delta Ct method. Microarray data was correlated with qPCR results using Pearson’s analyses and the same four samples on each line.