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. 2014 Feb 5;9(2):e88301. doi: 10.1371/journal.pone.0088301

Figure 3. PIM2 interacts with HIF-1α.

Figure 3

(A and B) HEK293T cells were transfected Flag-tagged PIM2 with GFP-tagged HIF-1α for 24 h and cultured under hypoxia for a further 24 h. Co-IP assays were performed with anti-Flag (A) or anti-GFP (B) antibodies, followed by immunoblot assays. (C) HEK293T cells were transfected Flag-tagged PIM2 for 24 h and cultured under hypoxia for a further 24 h. Co-IP assays were performed with anti-Flag antibody, followed by immunoblot assays. (D) HepG2 cells were cultured under hypoxia for 24 h. Co-IP assays were performed with anti-HIF-1α antibody, followed by immunoblot assays.