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. 2014 Feb 5;9(2):e88301. doi: 10.1371/journal.pone.0088301

Figure 5. PIM2 augments glycolysis and cell survival under hypoxia.

Figure 5

(A and B) HepG2 cells were transfected with empty vector or Flag-tagged PIM2 for 24 h and cultured under normoxia or hypoxia for a further 12–16 h. Levels of glucose (A) or lactate (B) in mediums were determined. (C and D) HepG2 cells were transfected with scramble siRNA or PIM2 siRNA for 24 h and cultured under normoxia or hypoxia for a further 12–16 h. Levels of glucose (C) or lactate (D) in mediums were determined. (E). HepG2 cells were transfected with scramble siRNA or PIM2 siRNA. After 24 h, the cells were re-plated and cultured under normoxia or hypoxia for a further 72 h. Cell numbers were counted every 24 h for the analysis of cell proliferation. (F) HepG2 cells were transfected with scramble siRNA or PIM2 siRNA. After 24 h, the cells were re-plated and cultured under normoxia or hypoxia for a further 24 h. The apoptosis changes were determined using the flow cytometry BD FaCSAria3 apparatus. All data represent the means ± SEM of three independent experiments, *p<0.05, **p<0.01.