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. 2014 Feb 5;9(2):e88398. doi: 10.1371/journal.pone.0088398

Figure 6. Neospora RNA elicits a TLR3-dependent type I interferon response.

Figure 6

a) QPCR analysis of Irf7 gene expression in wild-type mouse bone marrow-derived macrophages pretreated for 1 hr with either DMSO (control) or 100 nM bafilomycin A1 prior to 24 hr infection with live (Nc) or heat-killed Neospora (HK Nc). b) Irf7 expression in Myd88−/− macrophages incubated with 1 µg/ml of either poly(I:C) or total RNA from HFF cells (host), or Neospora parasites, either alone (white) or in complex with DOTAP transfection reagent (striped), to enhance targeting of RNA to endosomes. c) Irf7 expression in Myd88−/− macrophages incubated with 1 µg/ml of either Toxoplasma or Neospora RNA with DOTAP in cells pretreated with either DMSO or a small molecule inhibitor of TLR3/dsRNA complex formation. Error bars indicate standard deviations for three biological replicates; * = P≤0.01. Experiments were repeated two or three times with similar results.