Figure 2. Silencing PKCα and ε, but not PKC δ and θ, by siRNA reduces Nox5 derived superoxide production.

HEK cells stably expressing Nox5 were transfected with negative control siRNA or siRNA targeting PKCα, ε, δ, θ for 48 h. Lysates were immunoblotted for PKCα, ε, δ, θ, Nox5 and GAPDH as loading control (A, C, E). Superoxide production was measured using L-012 chemiluminescence in response to PMA (100 nM) stimulation (B, D, F) (means ± S.E., n = 6). Results are representative of at least 3–5 separate experiments, presented as means ± S.E., * p<0.05 versus Vehicle.