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. 2014 Feb 5;9(2):e88405. doi: 10.1371/journal.pone.0088405

Figure 3. A constitutively active form of PKCα increases basal and stimulated Nox5 activity.

Figure 3

HEK cells stably expressing Nox5 were transfected with either control plasmid (RFP) or myr-PKCα (A), myr-PKCε, PKCε A159E (D), and cell lysates were immunoblotted with V5 and HA antibodies. Basal (B, E) and PMA stimulated (C, F) superoxide production were measured using L-012 chemiluminescence. (means ± S.E., n = 6–8). Results are representative of at least 3 separate experiments, presented as means ± S.E., * p<0.05 versus Vehicle.