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. 2014 Feb 5;9(2):e88327. doi: 10.1371/journal.pone.0088327

Figure 1. Expression of constructs and T cell expansion.

Figure 1

A, B and D) Human moDCs were transduced with either the non-ubiquitinated (0x) or ubiquitinated (1x) HIV-1 CN54 gag constructs for 24 h or untransduced (control) and then cultured for a further 18 h in the presence (grey bar) or absence (hatched bar) of the proteasome inhibitor, MG132 (A and B). Cells were then harvested and stained intracellularly with anti-gag-PE antibody and analysed for the frequency (A) and density (B) of gag by flow cytometry. Results shown are the mean±SD for 3 donors and representative flow cytometry plots are shown in D. C) MoDCs were transduced with either the non-ubiquitinated (0x, closed circle) or ubiquitinated (1x, closed square) HIV-1 CN54 gag constructs and co-cultured with autologous naïve T cells. On a weekly basis, cells were harvested and counted by Trypan blue exclusion and co-cultured with freshly transduced moDCs. Graph shows the mean±SD of 4 donors.