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. 2014 Feb 5;9(2):e82624. doi: 10.1371/journal.pone.0082624

Figure 3. Gel analysis of RNA-primed MPRCA products to detect the contaminating DNA in Phi29 DNA polymerase.

Figure 3

(A) DNA amplification with Phi29 DNA polymerase prepared by our procedure. (B) DNA amplification with commercially available Phi29 DNA polymerase (Epicentre, Lot No. 10710). (C) DNA amplification with Phi29 DNA polymerase prepared by DNase-treatment. All gel images show the BamHI/EcoRI double digested amplification products. Black arrow indicates the specific amplification products from pUC19; M, AccuRuler 1-kb DNA RTU ladder (Maestrogen). All reactions were repeated three times or more; typical results are shown.