Figure 5. Clonal isolation after gene transfer in CMK6_SFF cells.

The plasmid pCAG-GFP2, which carries the CAG promoter-driven GFP2 green fluorescent protein cDNA and a neomycin resistance gene, was used for the gene transfer experiment. CMK6_SFF cells were transfected with pCAG-GFP2 using Human Stem Cell Nucleofector Solution 1 (Amaxa) and the A-013 program following the manufacturer's instructions. After 7–10 days of the gene transfer, G418-resistant colonies derived from single cells were selected and propagated in the MT-fCFA medium containing 80 µg/ml G418.