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CMK6SFF cells were treated in the MT-CDM medium in the presence or absence of 10 µM SB, 1 µM PD, and 1 µM DM for 4 days. A combination of SB and PD and/or DM in the MT-CDM medium generated the cells that highly expressed the neuroectoderm markers Sox1 and/or Pax6. SB, SB431542 (TGF-β inhibitor). PD, PD0325901 (MEK inhibitor). DM, dorsomorphin (BMP inhibitor). Scale bar = 100 µm.
