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. 2014 Feb 5;9(2):e88346. doi: 10.1371/journal.pone.0088346

Table 1. Passage frequency and split ratio of monkey ESCs grown under the MT-fCFA culture condition.

Cell Culture condition Passaging Frequency (Days) Split Ratio
CMK6 20% KSR, DMEM/F12, Colony on MEF 3–4 1∶4–5
CMK6SFF MT-fCFA, Single-cell, Feeder-free 2–3 1∶4–8
CMK970 MT-fCFA, Single-cell, Feeder-free 2–3 1∶5–10

CMK6SFF indicates the CMK6 cells adapted to single-cell and feeder-free condition. CMK970 is a cell line established under the SFF condition immediately after ICM isolation.