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. Author manuscript; available in PMC: 2014 Apr 17.
Published in final edited form as: Nature. 2013 Sep 11;502(7471):393–396. doi: 10.1038/nature12585

Extended Data Figure 8. Requirement for the Polδ subunit Pol32 in DNA extension, and interaction of Pif1 with PCNA.

Extended Data Figure 8

Purified Polδ (FLAG-Pol3, GST-Pol31, Pol32), Polδ* (FLAG-Pol3, GST-Pol31) and MBP-Pol32 were analyzed by SDS-PAGE and staining with Coomassie Blue. b. Pulldown assay to examine Pol32-Polδ* interaction. c. DNA synthesis was performed with Polδ or Polδ* (20 or 40 nM) with Pif1 (40 nM). In lanes 10–13, Polδ* and Pol32 (125 nM) were preincubated on ice for 10 min before use. The reaction products from the 8-min timepoint were resolved in a native gel (left) or denaturing gel (right). d. Pulldown reactions of 6XHis-Pif1 and PCNA (left), Polδ (FLAG-Pol3, GST-Pol31, Pol32) and PCNA, Polδ and Pif1 (right).