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Expression of CXCR4 in the parental low metastatic human osteosarcoma HOS cell line and the highly metastatic 143B subline. a semi-quantitative RT/PCR analysis of Cxcr4 and Gapdh (reference) transcript levels in total RNA isolated from 50 % (a) and 100 % (b) confluent cells, M marker lanes. b FACS analysis of indicated cell lines processed for cell surface immunostaining of CXCR4 in the absence (control) or presence (CXCR4+) of primary 12G5 CXCR4 antibodies
