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. 2014 Jan 18;4(3):267–279. doi: 10.7150/thno.7323

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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In vitro characterization of the drug-resistant cancer cell lines. (A) Clonogenic survival assay of the drug-resistant and the parental cell lines challenged with drug (10µM of tamoxifen; 250nM paclitaxel; 300nM doxorubicin). (B) Matrigel invasion assays comparing the ability of the drug-resistant cells lines to invade through matrigel to the parental. (C) Quantitative PCR analysis of breast cancer cell lines expressing uPAR normalized to normal human mammary epithelial cells. The cell lines analyzed included representative cell lines of each breast cancer subtype in addition to the drug-resistant variants.