Figure 4.

SPECT/CT imaging reveals that ¹¹¹In-2G10 localizes to the tumor interior of the uPAR expressing drug-resistant xenografts preferentially. SCID mice bearing bilateral orthotopic xenograft tumors were injected with ¹¹¹In-DOTA-labeled 2G10. Each animal (n=3 /xenograft/probe) received an injected dose of 2.5 - 3.8 ug of radiolabeled antibody corresponding to an activity of 230 - 290 µCi. Transverse, coronal and sagittal views of co-registered SPECT/CT images depict ¹¹¹In-2G10 localizing to the two tumors (Tu-1 and Tu-2) in the MCF-7 TamR, MDA-MB-231 TaxR and MDA-MB-231 DoxR xenografts. Secondary hepatic uptake of the probe is visible MCF-7 TamR, MDA-MB-231, and MDA-MB-231 TaxR. No localization of ¹¹¹In-2G10 is observed in the MCF-7 xenograft with only hepatic uptake present. The images presented here were acquired 72 hours post-injection and are representative of n = 3 mice imaged/xenograft.