Figure 5.

Biodistribution data for (A) MCF-7 and MCF-7 TamR (TamR); (B) MDA-MB-231 (231) and MDA-MB-231 (uPAR-): (C) MDA-MB-231 DoxR (DoxR); and (D) MDA-MDA-231 TaxR (TaxR). Mice (n = 3 mice for each time point per xenograft) were injected with 25 µCi of ¹¹¹In-2G10 via tail vein. Tissues were harvested at 24, 48 and 72hr post-injection and analyzed. In addition to ¹¹¹In-2G10, mice (n = 3 mice per xenograft) were injected with ¹¹¹In-A11 IgG (A11 IgG) to assess the level of non-specific tumor uptake contributed by the EPR effect at 72hr post-injection of A11 IgG. Tumor uptake was also blocked in uPAR expressing xenografts by i.v. pre-injection of 200 µg of cold 2G10 IgG (Block) prior to probe administration and uptake was analyzed 72hr post-injection.